Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94514

SGTA (phospho-Ser305) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SGTA (phospho-Ser305) rabbit pAb from ELK Biotechnology Read more ›

SGTA (phospho-Ser305) rabbit pAb
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SGTA (phospho-Ser305) rabbit pAb

Product Description

SGTA (phospho-Ser305) rabbit pAb is a research-use antibody from ELK Biotechnology for studying SGTA in WB, IHC, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human SGTA (phospho-Ser305)
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: SGTA
Collaborator ELK Biotechnology
Partner model ES13130
Target SGTA
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human SGTA (Ser305)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitous.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
SGTA
Also known as
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (Alpha-SGT) (Vpu-binding protein) (UBP)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHCELISA
Immunogen
Synthesized phosho peptide around human SGTA (Ser305)
Observed band
35kD
Cellular localization
Cytoplasm . Nucleus . Co-localizes with HSP90AB1 in the cytoplasm. Increased nuclear accumulation seen during cell apoptosis. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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