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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94500

SIDT2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SIDT2 rabbit pAb from ELK Biotechnology Read more ›

SIDT2 rabbit pAb
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SIDT2 rabbit pAb

Product Description

SIDT2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SIDT2 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of SIDT2 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody› Target: SIDT2
Collaborator ELK Biotechnology
Partner model ES13106
Target SIDT2
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human SIDT2 AA range: 393-443
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 —
Mouse — —
Rat — —
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
SIDT2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCELISA
Immunogen
Synthesized peptide derived from human SIDT2 AA range: 393-443
Cellular localization
Lysosome membrane ; Multi-pass membrane protein . Cell membrane . Mainly localizes to lysosomes and only partly to the plasma membrane (PubMed:28277980). Lysosomal localization is required for SIDT2-mediated intracellular degradation of endogenous RNA (By similarity). .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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