eIF2α (phospho Ser51) rabbit pAb from ELK Biotechnology Read more ›
eIF2α (phospho Ser51) rabbit pAb is a research-use antibody from ELK Biotechnology for studying eIF2α in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Dog, Pig, Fish. Specifications and supporting documents below should be reviewed when planning the experiment.
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],
| Specificity | Phospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1304 |
| Target | eIF2α |
| Research areas | >>Autophagy - animal, >>Protein processing in endoplasmic reticulum, >>Apoptosis, >>Non-alcoholic fatty liver disease, >>Alzheimer disease, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Hepatitis C, >>Measles, >>Influenza A, >>Herpes simplex virus 1 infection, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse,Rat,Dog,Pig,Fish |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51. AA range:21-70 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B cells,Brain,Fibroblast,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Dog | |||||||
| Pig | |||||||
| Fish |
| Species | Dilution | Notes |
|---|---|---|
| Human | IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Dog | — | — |
| Pig | — | — |
| Fish | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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