Primary Antibody ISO 9001|ABMIUM Verified™
ABM94456

SOLH2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SOLH2 rabbit pAb from ELK Biotechnology Read more ›

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SOLH2 rabbit pAb
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SOLH2 rabbit pAb

Product Description

SOLH2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving SOLH2.

Background

This gene encodes one of testis-specific transcription factors which are essential for spermatogenesis, oogenesis and folliculogenesis. This gene is located on chromosome 13. The proteins encoded by this gene and another testis-specific transcription factor, SOHLH1, can form heterodimers, in addition to homodimers. There is a read-through locus (GeneID: 100526761) that shares sequence identity with this gene and the upstream CCDC169 (GeneID: 728591). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013],

Additional antibody information

Specificity This antibody detects endogenous levels of SOLH2 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: SOLH2
Target SOLH2
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300
Species reactivity Human; Mouse;Rat
Cellular localization Nucleus . Cytoplasm . Translocates from the cytoplasm into the nucleus and the translocation is dependent on SOHLH1 expression. .
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHC
Dilution range WB 1:500-2000;IHC-p 1:50-300
Immunogen Synthesized peptide derived from human SOLH2 AA range: 117-167
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
SOLH2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from human SOLH2 AA range: 117-167
Cellular localization
Nucleus . Cytoplasm . Translocates from the cytoplasm into the nucleus and the translocation is dependent on SOHLH1 expression. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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