Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94433

SPS1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SPS1 rabbit pAb from ELK Biotechnology Read more ›

SPS1 rabbit pAb
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SPS1 rabbit pAb

Product Description

SPS1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SPS1 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010],

Additional antibody information

Specificity This antibody detects endogenous levels of SPS1 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: SPS1
Collaborator ELK Biotechnology
Partner model ES12980
Target SPS1
Research areas>>Selenocompound metabolism, >>Metabolic pathways
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human SPS1 AA range: 323-373
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Isoform 1: Gradually expressed during the cell cycle until G2/M phase and then decreases. ; Isoform 2: Gradually expressed during the cell cycle until G2/M phase and then decreases. ; Isoform 3: Gradually expressed during the cell cycle until S phase and then decreases.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
SPS1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human SPS1 AA range: 323-373
Cellular localization
Isoform 1: Cell membrane ; Peripheral membrane protein . Nucleus membrane ; Peripheral membrane protein .; Isoform 2: Cytoplasm .; Isoform 3: Cytoplasm .; Isoform 4: Cytoplasm .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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