ABM94419
SREBP-1 (Acetyl-Lys324) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
SREBP-1 (Acetyl-Lys324) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
SREBP-1 (Acetyl-Lys324) rabbit pAb is an ELK Biotechnology antibody for research workflows involving SREBP-1.
Background
This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Mar 2016],
Additional antibody information
| Specificity | This antibody detects endogenous acetyl levels of SREBP-1 (Acetyl-Lys324) at Human:K324, Mouse:K318, Rat:K318 |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: SREBP-1
| Target | SREBP-1 |
| Also known as | Sterol regulatory element-binding protein 1 (SREBP-1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1) Cleaved into: Processed sterol regulatory element-binding protein 1 |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-200, WB 1:500-2000 |
| Species reactivity | Human; Mouse; Rat |
| Observed band | 130kD |
| Cellular localization | Sterol regulatory element-binding protein 1: Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein . At high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum. Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed. .; Processed sterol regulatory element-binding protein 1: Nucleus .; Isoform SREBP-1aDelta: Nucleus .; Isoform SREBP-1cDelta: Nucleus . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>AMPK signaling pathway;>>Insulin signaling pathway;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Alcoholic liver disease |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | IHC-p 1:50-200, WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human SREBP-1 (Acetyl-Lys324) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-200 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
Specifications
Target
SREBP-1
Also known as
Sterol regulatory element-binding protein 1 (SREBP-1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1) Cleaved into: Processed sterol regulatory element-binding protein 1
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-200, WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse; Rat
Tested applications
WBIHCIF
Immunogen
Synthesized peptide derived from human SREBP-1 (Acetyl-Lys324)
Observed band
130kD
Cellular localization
Sterol regulatory element-binding protein 1: Endoplasmic reticulum membrane ; Multi-pass membrane protein . Golgi apparatus membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane ; Multi-pass membrane protein . At high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum. Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed. .; Processed sterol regulatory element-binding protein 1: Nucleus .; Isoform SREBP-1aDelta: Nucleus .; Isoform SREBP-1cDelta: Nucleus .
Dilution range
IHC-p 1:50-200, WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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