Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94418

SRGN rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SRGN rabbit pAb from ELK Biotechnology Read more ›

SRGN rabbit pAb
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SRGN rabbit pAb

Product Description

SRGN rabbit pAb is a research-use antibody from ELK Biotechnology for studying SRGN in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. [provided by RefSeq, Jul 2010],

Additional antibody information

Specificity This antibody detects endogenous levels of SRGN at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: SRGN
Collaborator ELK Biotechnology
Partner model ES12956
Target SRGN
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human SRGN AA range: 30-80
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat

Specifications

Target
SRGN
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human SRGN AA range: 30-80
Cellular localization
Cytoplasmic granule . Cytolytic granule . Secreted, extracellular space . Golgi apparatus . Found in mast cell granules and in cytoplasmic granules of cytolytic T lymphocytes from where it is secreted upon cell activation (By similarity). Secreted constitutively by endothelial cells and macrophages (PubMed:11154222). Located to Golgi apparatus during neutrophil differentiation (PubMed:15136585). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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