c-Src (phospho Tyr529) rabbit pAb from ELK Biotechnology Read more ›
c-Src (phospho Tyr529) rabbit pAb is a research-use antibody from ELK Biotechnology for studying c-Src in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-c-Src (Y529) Polyclonal Antibody detects endogenous levels of c-Src protein only when phosphorylated at Y529. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of COS7 cells using Phospho-c-Src (Y529) Polyclonal Antibody diluted at 1:2000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Src (Phospho-Tyr529) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Src (Phospho-Tyr529) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1293 |
| Target | c-Src |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>ErbB signaling pathway, >>Rap1 signaling pathway, >>Chemokine signaling pathway, >>Mitophagy - animal, >>Endocytosis, >>Axon guidance, >>VEGF signaling pathway, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Gap junction, >>Platelet activation, >>Neutrophil extracellular trap formation, >>C-type lectin receptor signaling pathway, >>GABAergic synapse, >>Inflammatory mediator regulation of TRP channels, >>Regulation of actin cytoskeleton, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Prolactin signaling pathway, >>Thyroid hormone signaling pathway, >>Oxytocin signaling pathway, >>Relaxin signaling pathway, >>Bacterial invasion of epithelial cells, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Yersinia infection, >>Tuberculosis, >>Hepatitis B, >>Human cytomegalovirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 inf |
| Reactivity | Human,Mouse,Rat,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Src around the phosphorylation site of Tyr529. AA range:487-536 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues.; Isoform 1: Expressed in spleen and liver. ; Isoform 2: Expressed in brain. ; Isoform 3: Expressed in brain. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Monkey | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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