Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94395

STAU2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

STAU2 rabbit pAb from ELK Biotechnology Read more ›

STAU2 rabbit pAb 50µL/100µL
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STAU2 rabbit pAb 50µL/100µL

Product Description

STAU2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying STAU2 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Staufen homolog 2 is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. Staufen homolog 2 shares 48.5% and 59.9% similarity with drosophila and human staufen, respectively. The exact function of Staufen homolog 2 is not known, but since it contains 3 copies of conserved dsRNA binding domain, it could be involved in double-stranded RNA binding events. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009],

Additional antibody information

Specificity This antibody detects endogenous levels of STAU2 at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: STAU2
Collaborator ELK Biotechnology
Partner model ES12908
Target STAU2
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human STAU2 AA range: 75-125
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Amygdala,Brain,Cerebellum,Epithelium,Ovary,PCR rescued clones,Placenta,Retina,Teratocarcinoma,Thalamus,Thymus,Trachea,Urinary bladder carcinoma,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat

Specifications

Target
STAU2
Also known as
Double-stranded RNA-binding protein Staufen homolog 2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human STAU2 AA range: 75-125
Observed band
65kD
Cellular localization
Cytoplasm. Nucleus. Nucleus, nucleolus. Endoplasmic reticulum. Shuttles between the nucleolus, nucleus and the cytoplasm. Nuclear export of isoform 1 is independent of XPO1/CRM1 and requires the exportin XPO5. Nuclear export of isoform 2 and isoform 3 can occur by both XPO1/CRM1-dependent and XPO1/CRM1-independent pathways. Found in large cytoplasmic ribonucleoprotein (RNP) granules which are present in the actin rich regions of myelinating processes and associated with microtubules, polysomes and the endoplasmic reticulum. Also recruited to stress granules (SGs) upon inhibition of translation or oxidative stress. These structures are thought to harbor housekeeping mRNAs when translation is aborted (By similarity). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.187% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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