STX3 rabbit pAb from ELK Biotechnology Read more ›
STX3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying STX3 in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The gene is a member of the syntaxin family. The encoded protein is targeted to the apical membrane of epithelial cells where it forms clusters and is important in establishing and maintaining polarity necessary for protein trafficking involving vesicle fusion and exocytosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010],
| Specificity | This antibody detects endogenous levels of STX3 at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night |
| Collaborator | ELK Biotechnology |
| Partner model | ES12889 |
| Target | STX3 |
| Research areas | >>SNARE interactions in vesicular transport, >>Synaptic vesicle cycle |
| Reactivity | Human,Mouse,Rat |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from human STX3 AA range: 6-56 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform A: Expressed in small intestine, kidney, pancreas, placenta as well as in retina. Weaker expression in lung, liver and heart. Not expressed in brain and skeletal muscle. ; Isoform B: Expressed only in the retina. ; Isoform 3: Ubiquitously expressed. |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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