TIM50 rabbit pAb
TIM50 rabbit pAb from ELK Biotechnology Read more ›
Product Description
TIM50 rabbit pAb is an ELK Biotechnology antibody for research workflows involving TIM50.
Background
This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis. [provided by RefSeq, Jul 2016],
Additional antibody information
| Specificity | This antibody detects endogenous levels of TIM50 at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
| Target | TIM50 |
| Also known as | Mitochondrial import inner membrane translocase subunit TIM50 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human; Mouse |
| Observed band | 38kD |
| Cellular localization | Mitochondrion inner membrane ; Single-pass membrane protein .; Isoform 2: Nucleus speckle . Nuclear and enriched in speckles with snRNPs. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.43% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human TIM50 AA range: 109-159 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.