Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94205

TORC1/CRTC1 (phospho-Ser151) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TORC1/CRTC1 (phospho-Ser151) rabbit pAb from ELK Biotechnology Read more ›

TORC1/CRTC1 (phospho-Ser151) rabbit pAb
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TORC1/CRTC1 (phospho-Ser151) rabbit pAb

Product Description

TORC1/CRTC1 (phospho-Ser151) rabbit pAb is a research-use antibody from ELK Biotechnology for studying TORC1 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human TORC1 or CRTC1 (phospho-Ser151)
Validation evidence captions Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

Key Facts

Product typePrimary Antibody Target: TORC1
Collaborator ELK Biotechnology
Partner model ES12606
Target TORC1
Research areas>>Human T-cell leukemia virus 1 infection
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human TORC1 and CRTC1 (Ser151)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Rat
Mouse

Specifications

Target
TORC1
Also known as
CREB-regulated transcription coactivator 1 (Mucoepidermoid carcinoma translocated protein 1) (Transducer of regulated cAMP response element-binding protein 1) (TORC-1) (Transducer of CREB protein 1)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthesized phosho peptide around human TORC1 and CRTC1 (Ser151)
Observed band
70kD
Cellular localization
Cytoplasm . Nucleus . Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity). .
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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