Trk A (Phospho-Y496) Rabbit pAb from ELK Biotechnology Read more ›
Trk A (Phospho-Y496) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Trk A in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008],
| Specificity | This antibody detects endogenous pospho levels of human Trk A (Phospho-Y496) |
|---|---|
| Validation evidence captions | Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour. |
| Collaborator | ELK Biotechnology |
| Partner model | ES12561 |
| Target | Trk A |
| Research areas | >>MAPK signaling pathway, >>Ras signaling pathway, >>Calcium signaling pathway, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Neurotrophin signaling pathway, >>Inflammatory mediator regulation of TRP channels, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Thyroid cancer, >>Central carbon metabolism in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000; IF/ICC 1:100-500; ELISA 1:5000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized pospho peptide derived from human Trk A (Phospho-Y496) |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. |
| Purity | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000; IF/ICC 1:100-500; ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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