Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94122

UBC rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

UBC rabbit pAb from ELK Biotechnology Read more ›

UBC rabbit pAb
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UBC rabbit pAb

Product Description

UBC rabbit pAb is a research-use antibody from ELK Biotechnology for studying UBC in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010],

Additional antibody information

Specificity This antibody detects endogenous levels of UBC at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: UBC
Collaborator ELK Biotechnology
Partner model ES12457
Target UBC
Research areas>>PPAR signaling pathway, >>Ubiquitin mediated proteolysis, >>Mitophagy - animal, >>Parkinson disease, >>Pathways of neurodegeneration - multiple diseases, >>Shigellosis, >>Kaposi sarcoma-associated herpesvirus infection
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human UBC AA range: 160-210
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat

Specifications

Target
UBC
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human UBC AA range: 160-210
Cellular localization
Ubiquitin: Cytoplasm . Nucleus . Mitochondrion outer membrane ; Peripheral membrane protein .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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