ABM94020
XYLT1 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
XYLT1 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
XYLT1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving XYLT1.
Background
This locus encodes a xylosyltransferase enzyme. The encoded protein catalyzes transfer of UDP-xylose to serine residues of an acceptor protein substrate. This transfer reaction is necessary for biosynthesis of glycosaminoglycan chains. Mutations in this gene have been associated with increased severity of pseudoxanthoma elasticum.[provided by RefSeq, Nov 2009],
Additional antibody information
| Specificity | This antibody detects endogenous levels of XYLT1 at Human/Mouse/Rat |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: XYLT1
| Target | XYLT1 |
| Also known as | Xylosyltransferase 1 (EC 2.4.2.26) (Peptide O-xylosyltransferase 1) (Xylosyltransferase I) (XT-I) (XylT-I) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human; Mouse;Rat |
| Observed band | 105kD |
| Cellular localization | Golgi apparatus membrane ; Single-pass type II membrane protein . Secreted . Detected predominantly in the Golgi apparatus. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.253% sodium azide. |
| Research area | >>Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate;>>Glycosaminoglycan biosynthesis - heparan sulfate / heparin;>>Metabolic pathways |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human XYLT1 AA range: 312-362 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Target
XYLT1
Also known as
Xylosyltransferase 1 (EC 2.4.2.26) (Peptide O-xylosyltransferase 1) (Xylosyltransferase I) (XT-I) (XylT-I)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.253% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse;Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human XYLT1 AA range: 312-362
Observed band
105kD
Cellular localization
Golgi apparatus membrane ; Single-pass type II membrane protein . Secreted . Detected predominantly in the Golgi apparatus. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.253% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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