ABM93840
ZPBP1 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ZPBP1 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ZPBP1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving ZPBP1.
Background
ZPBP is one of several proteins that are thought to participate in secondary binding between acrosome-reacted sperm and the egg-specific extracellular matrix, the zona pellucida (McLeskey et al., 1998 [PubMed 9378618]).[supplied by OMIM, Aug 2008],
Additional antibody information
| Specificity | This antibody detects endogenous levels of ZPBP1 at Human/Mouse |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: ZPBP1
| Target | ZPBP1 |
| Also known as | Zona pellucida-binding protein 1 (Sp38) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 |
| Species reactivity | Human; Mouse |
| Observed band | 38kD |
| Cellular localization | Cytoplasmic vesicle, secretory vesicle, acrosome membrane ; Peripheral membrane protein . Secreted . First localized in acrosome granule, later migrates to the inner and outer acrosomal membrane. Released after the acrosomal reaction. . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.15% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:500-2000 |
| Immunogen | Synthesized peptide derived from human ZPBP1 AA range: 176-226 |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
Specifications
Target
ZPBP1
Also known as
Zona pellucida-binding protein 1 (Sp38)
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.15% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human; Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human ZPBP1 AA range: 176-226
Observed band
38kD
Cellular localization
Cytoplasmic vesicle, secretory vesicle, acrosome membrane ; Peripheral membrane protein . Secreted . First localized in acrosome granule, later migrates to the inner and outer acrosomal membrane. Released after the acrosomal reaction. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.15% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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