Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93807

TRPA1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TRPA1 rabbit pAb from ELK Biotechnology Read more ›

TRPA1 rabbit pAb
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TRPA1 rabbit pAb

Product Description

TRPA1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TRPA1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

transient receptor potential cation channel subfamily A member 1(TRPA1) Homo sapiens The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity TRPA1 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

Key Facts

Product typePrimary Antibody Target: TRPA1
Collaborator ELK Biotechnology
Partner model ES11963
Target TRPA1
Research areas>>Inflammatory mediator regulation of TRP channels
Reactivity Human,Mouse,Rat
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed at very low levels in fibroblasts.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TRPA1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIFELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
123kD
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Dilution range
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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