Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93761

MUC3A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MUC3A rabbit pAb from ELK Biotechnology Read more ›

MUC3A rabbit pAb 50µL/100µL
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MUC3A rabbit pAb 50µL/100µL

Product Description

MUC3A rabbit pAb is a research-use antibody from ELK Biotechnology for studying MUC3A in IHC, IF workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation (Gendler and Spicer, 1995 [PubMed 7778880]).[supplied by OMIM, Aug 2008],

Additional antibody information

Specificity MUC3A Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: MUC3A
Collaborator ELK Biotechnology
Partner model ES11883
Target MUC3A
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IHC,IF
ConjugationUnconjugated
Dilution rangeIHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Broad specificity; small intestine, colon, colonic tumors, heart, liver, thymus, prostate, pancreas and gall bladder.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC-p 1:50-300
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes

Specifications

Target
MUC3A
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHCIF
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
279kD
Cellular localization
Isoform 1: Membrane; Single-pass membrane protein .; Isoform 2: Secreted .; Isoform 3: Secreted .; Isoform 4: Secreted .; Isoform 5: Secreted.
Dilution range
IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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