ABM93739
HLAG rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HLAG rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HLAG rabbit pAb is an ELK Biotechnology antibody for research workflows involving HLAG.
Background
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | HLAG Polyclonal Antibody detects endogenous levels of protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: HLAG
| Target | HLAG |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 ELISA 1:5000-20000 |
| Species reactivity | Human |
| Observed band | 37kD |
| Cellular localization | Isoform 1: Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane . Early endosome membrane .; Soluble HLA class I histocompatibility antigen, alpha chain G: Secreted .; Isoform 2: Cell membrane ; Single-pass type I membrane protein .; Isoform 3: Cell membrane ; Single-pass type I membrane protein .; Isoform 4: Cell membrane ; Single-pass type I membrane protein .; Isoform 5: Secreted . Early endosome .; Isoform 6: Secreted .; Isoform 7: Secreted .; Cell projection, filopodium membrane . HLA-G trogocytosis from extravillous trophoblast's filopodia occurs in the majority of decidual NK cells. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Research area | >>Endocytosis;>>Phagosome;>>Cellular senescence;>>Cell adhesion molecules;>>Antigen processing and presentation;>>Type I diabetes mellitus;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Viral carcinogenesis;>>Autoimmune thyroid disease;>>Allograft rejection;>>Graft-versus-host disease;>>Viral myocarditis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000 ELISA 1:5000-20000 |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 ELISA 1:5000-20000 | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
HLAG
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
37kD
Cellular localization
Isoform 1: Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane . Early endosome membrane .; Soluble HLA class I histocompatibility antigen, alpha chain G: Secreted .; Isoform 2: Cell membrane ; Single-pass type I membrane protein .; Isoform 3: Cell membrane ; Single-pass type I membrane protein .; Isoform 4: Cell membrane ; Single-pass type I membrane protein .; Isoform 5: Secreted . Early endosome .; Isoform 6: Secreted .; Isoform 7: Secreted .; Cell projection, filopodium membrane . HLA-G trogocytosis from extravillous trophoblast's filopodia occurs in the majority of decidual NK cells. .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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