Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93457

FPR2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

FPR2 rabbit pAb from ELK Biotechnology Read more ›

FPR2 rabbit pAb
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FPR2 rabbit pAb

Product Description

FPR2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying FPR2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse, Swine. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4).,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expressed abundantly in the lung and neutrophils. Also found in the spleen and testis.,

Additional antibody information

Specificity FPR2 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: FPR2
Collaborator ELK Biotechnology
Partner model ES11464
Target FPR2
Research areas>>Neuroactive ligand-receptor interaction, >>Neutrophil extracellular trap formation, >>Staphylococcus aureus infection
Reactivity Human,Rat,Mouse,Swine
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human protein . at AA range: 130-210
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Detected in lung, bone marrow, neutrophils, spleen and testis.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

4 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Swine
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Rat
Mouse
Swine
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
FPR2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse,Swine
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human protein . at AA range: 130-210
Observed band
38kD
Cellular localization
Cell membrane ; Multi-pass membrane protein. Associates with Amyloid-beta protein 42, product of APP, at the cell surface and the complex is then rapidly internalized (PubMed:11689470). Also internalized in the presence of humanin (PubMed:15465011). .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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