Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93451

BKRB2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

BKRB2 rabbit pAb from ELK Biotechnology Read more ›

BKRB2 rabbit pAb 50µL/100µL
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BKRB2 rabbit pAb 50µL/100µL

Product Description

BKRB2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying BKRB2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity BKRB2 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: BKRB2
Collaborator ELK Biotechnology
Partner model ES11454
Target BKRB2
Research areas>>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>Sphingolipid signaling pathway, >>Neuroactive ligand-receptor interaction, >>Complement and coagulation cascades, >>Inflammatory mediator regulation of TRP channels, >>Regulation of actin cytoskeleton, >>Endocrine and other factor-regulated calcium reabsorption, >>Chagas disease, >>Pathways in cancer
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human protein . at AA range: 140-220
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitous. Widespread in normal smooth muscle tissue and neurons.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
BKRB2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human protein . at AA range: 140-220
Observed band
43kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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