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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93433

SPXN rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SPXN rabbit pAb from ELK Biotechnology Read more ›

SPXN rabbit pAb
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SPXN rabbit pAb

Product Description

SPXN rabbit pAb is a research-use antibody from ELK Biotechnology for studying SPXN in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a hormone involved in modulation of cardiovascular and renal function. It has also been shown in rats to cause weight loss. Several transcript variants have been found for this gene. [provided by RefSeq, Feb 2016],

Additional antibody information

Specificity SPXN Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody› Target: SPXN
Collaborator ELK Biotechnology
Partner model ES11421
Target SPXN
Research areas>>Neuroactive ligand-receptor interaction
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human protein . at AA range: 1-50
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in the type I glomic cells within the carotid body (at protein level). Expressed predominantly in pancreas, testis, kidney, brain and placenta. Expressed in submucosal layer of esophagus and stomach fundus.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000 —
Rat — —
Mouse — —
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
SPXN
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human protein . at AA range: 1-50
Observed band
12kD
Cellular localization
Secreted. Secreted, extracellular space. Cytoplasmic vesicle, secretory vesicle. Secreted via the classical ER/Golgi-dependent pathway into the extracellular medium largely as a full-length protein without the signal peptide, and not as a hydrolyzed and amidated peptide (PubMed:19193193 and PubMed:17284679). Localized extracellularly surrounding the villous trophoblastic cells. Detected in the serum.
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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