Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93324

FGF3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

FGF3 rabbit pAb from ELK Biotechnology Read more ›

FGF3 rabbit pAb
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FGF3 rabbit pAb

Product Description

FGF3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying FGF3 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its similarity with mouse fgf3/int-2, a proto-oncogene activated in virally induced mammary tumors in the mouse. Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. Studies of the similar genes in mouse and chicken suggested the role in inner ear formation. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity FGF3 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: FGF3
Collaborator ELK Biotechnology
Partner model ES11252
Target FGF3
Research areas>>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>PI3K-Akt signaling pathway, >>Regulation of actin cytoskeleton, >>Pathways in cancer, >>Chemical carcinogenesis - receptor activation, >>Melanoma, >>Breast cancer, >>Gastric cancer
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human protein . at AA range: 130-210
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Placenta,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
FGF3
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human protein . at AA range: 130-210
Observed band
26kD
Cellular localization
Secreted .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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