EF-1 α1/2 (Acetyl Lys41) rabbit pAb from ELK Biotechnology Read more ›
EF-1 α1/2 (Acetyl Lys41) rabbit pAb is a research-use antibody from ELK Biotechnology for studying EF-1 α1/2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008],
| Specificity | Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody detects endogenous levels of EF-1 α1/2 protein only when acetylated at K41. |
|---|---|
| Validation evidence captions | Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of A549 cells using Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from A549 cells, using EEF1A-pan (Acetyl-Lys41) Antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1103 |
| Target | EF-1 α1/2 |
| Research areas | >>Nucleocytoplasmic transport, >>Legionellosis, >>Leishmaniasis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from human EEF1A around the Acetylation site of Lys41. AA range:1-50 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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