Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93157

VWF rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

VWF rabbit pAb from ELK Biotechnology Read more ›

VWF rabbit pAb 50µL/100µL
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VWF rabbit pAb 50µL/100µL

Product Description

VWF rabbit pAb is a research-use antibody from ELK Biotechnology for studying VWF in IHC, IF workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015],

Additional antibody information

Specificity VWF Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

Key Facts

Product typePrimary Antibody Target: VWF
Collaborator ELK Biotechnology
Partner model ES10984
Target VWF
Research areas>>PI3K-Akt signaling pathway, >>Focal adhesion, >>ECM-receptor interaction, >>Complement and coagulation cascades, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Human papillomavirus infection, >>Coronavirus disease - COVID-19
Reactivity Human,Rat,Mouse
Applications IHC,IF
ConjugationUnconjugated
Dilution rangeIHC-p 1:50-300
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein AA range: 911-960
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Plasma.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC-p 1:50-300
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes

Specifications

Target
VWF
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHCIF
Immunogen
Synthesized peptide derived from part region of human protein AA range: 911-960
Observed band
309kD
Cellular localization
Secreted . Secreted, extracellular space, extracellular matrix . Localized to storage granules.
Dilution range
IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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