ABM93157
VWF rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
VWF rabbit pAb from ELK Biotechnology Read more ›
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Product Description
VWF rabbit pAb is an ELK Biotechnology antibody for research workflows involving VWF.
Background
This gene encodes a glycoprotein involved in hemostasis. The encoded preproprotein is proteolytically processed following assembly into large multimeric complexes. These complexes function in the adhesion of platelets to sites of vascular injury and the transport of various proteins in the blood. Mutations in this gene result in von Willebrand disease, an inherited bleeding disorder. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Oct 2015],
Additional antibody information
| Specificity | VWF Polyclonal Antibody detects endogenous levels of protein. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min). |
Key Facts
Product type
Primary Antibody›
Target: VWF
| Target | VWF |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-300 |
| Species reactivity | Human;Rat;Mouse |
| Observed band | 309kD |
| Cellular localization | Secreted . Secreted, extracellular space, extracellular matrix . Localized to storage granules. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Research area | >>PI3K-Akt signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Complement and coagulation cascades;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Human papillomavirus infection;>>Coronavirus disease - COVID-19 |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIF |
| Dilution range | IHC-p 1:50-300 |
| Immunogen | Synthesized peptide derived from part region of human protein AA range: 911-960 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
Specifications
Target
VWF
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse
Tested applications
IHCIF
Immunogen
Synthesized peptide derived from part region of human protein AA range: 911-960
Observed band
309kD
Cellular localization
Secreted . Secreted, extracellular space, extracellular matrix . Localized to storage granules.
Dilution range
IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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