NFκB-p65 (Acetyl Lys310) rabbit pAb from ELK Biotechnology Read more ›
NFκB-p65 (Acetyl Lys310) rabbit pAb is a research-use antibody from ELK Biotechnology for studying NFkB p65 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],
| Specificity | Acetyl-NFκB-p65 (K310) Polyclonal Antibody detects endogenous levels of NFκB-p65 protein only when acetylated at K310. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Hela cell. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-Tubulin Monoclonal Antibody(5G3)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,NFκB-p65 (Acetyl Lys310) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1094 |
| Target | NFkB p65 |
| Research areas | >>Antifolate resistance, >>MAPK signaling pathway, >>Ras signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>NF-kappa B signaling pathway, >>HIF-1 signaling pathway, >>Sphingolipid signaling pathway, >>Mitophagy - animal, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Longevity regulating pathway, >>Cellular senescence, >>Osteoclast differentiation, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>TNF signaling pathway, >>Neurotrophin signaling pathway, >>Prolactin signaling pathway, >>Adipocytokine signaling pathway, >>Relaxin signaling pathway, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>AGE-RAGE signaling pathway in diabe |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the acetylated site of Lys310. AA range:275-324 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Bone,Colon,Pancreas,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
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| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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