Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93059

KCC2B rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

KCC2B rabbit pAb from ELK Biotechnology Read more ›

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Product Description

KCC2B rabbit pAb is a research-use antibody from ELK Biotechnology for studying KCC2B in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],

Additional antibody information

Specificity KCC2B Polyclonal Antibody detects endogenous levels of protein.

Key Facts

Product typePrimary Antibody Target: KCC2B
Collaborator ELK Biotechnology
Partner model ES10807
Target KCC2B
Research areas>>ErbB signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>HIF-1 signaling pathway, >>Oocyte meiosis, >>Necroptosis, >>Adrenergic signaling in cardiomyocytes, >>Wnt signaling pathway, >>Axon guidance, >>Circadian entrainment, >>Long-term potentiation, >>Neurotrophin signaling pathway, >>Cholinergic synapse, >>Dopaminergic synapse, >>Olfactory transduction, >>Inflammatory mediator regulation of TRP channels, >>Insulin secretion, >>GnRH signaling pathway, >>Melanogenesis, >>Oxytocin signaling pathway, >>Glucagon signaling pathway, >>Aldosterone synthesis and secretion, >>Cushing syndrome, >>Gastric acid secretion, >>Parkinson disease, >>Pathways of neurodegeneration - multiple diseases, >>Amphetamine addiction, >>Tuberculosis, >>Pathways in cancer, >>Proteoglycans in cancer, >>Glioma, >>Diabetic cardiomyopathy, >>Lipid and atherosclerosis
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
KCC2B
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
73kD
Cellular localization
Cytoplasm, cytoskeleton . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Sarcoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, synapse . In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR.
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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