ABM93047
HA-tag rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
HA-tag rabbit pAb from ELK Biotechnology Read more ›
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Product Description
HA-tag rabbit pAb is an ELK Biotechnology antibody for research workflows involving HA-tag.
Background
The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.
Additional antibody information
| Specificity | HA-tag Polyclonal Antibody detects HA-tagged recombinant proteins or HA-tagged proteins overexpressed in cells. |
|---|---|
| Validation evidence captions | Western Blot analysis using HA-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing HA tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | IP analysis of HA-tag Polyclonal Antibody |
Key Facts
Product type
Primary Antibody›
Target: HA-tag
| Target | HA-tag |
| Also known as | HA epitope tag; hemagglutinin |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications. |
| Species reactivity | Species independent |
| Observed band | 15kD |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISAIP |
| Dilution range | Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications. |
| Immunogen | HA synthetic peptide conjugated to KLH. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Species independent |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Species independent | Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications. | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IP
| Species | Dilution | Notes |
|---|
Specifications
Target
HA-tag
Also known as
HA epitope tag; hemagglutinin
Concentration
1 mg/ml
Working concentration
Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Species independent
Tested applications
WBELISAIP
Immunogen
HA synthetic peptide conjugated to KLH.
Observed band
15kD
Dilution range
Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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