HA-tag rabbit pAb from ELK Biotechnology Read more ›
HA-tag rabbit pAb is a research-use antibody from ELK Biotechnology for studying HA-tag in WB, ELISA, IP workflows with listed reactivity for General. Specifications and supporting documents below should be reviewed when planning the experiment.
The HA tag (Y-P-Y-D-V-P-D-Y-A) is derived from an epitope (amino acids 98-106) of the influenza hemagglutinin (HA) protein. HA tag is used as a general epitope tag in expression vectors for many recombinant proteins. The presence of HA tag facilitates the detection, isolation and purification of recombinant fusion proteins.
| Specificity | HA-tag Polyclonal Antibody detects HA-tagged recombinant proteins or HA-tagged proteins overexpressed in cells. |
|---|---|
| Validation evidence captions | Western Blot analysis using HA-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing HA tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | IP analysis of HA-tag Polyclonal Antibody |
| Collaborator | ELK Biotechnology |
| Partner model | ES1077 |
| Target | HA-tag |
| Research areas | Antibody Research |
| Reactivity | General |
| Applications | WB,ELISA,IP |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | HA synthetic peptide conjugated to KLH. |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Species independent |
| Species | Dilution | Notes |
|---|---|---|
| Species independent | Western Blot: 1/1000 - 1/3000;. IP: 1:300 Not yet tested in other applications. | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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