Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93046

S35B2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

S35B2 rabbit pAb from ELK Biotechnology Read more ›

S35B2 rabbit pAb
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S35B2 rabbit pAb

Product Description

S35B2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying S35B2 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Sulfotransferases (e.g., SULT4A1; MIM 608359) use an activated form of sulfate, 3-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS), as a common sulfate donor for sulfation of glycoproteins, proteoglycans, and glycolipids in the endoplasmic reticulum and Golgi apparatus. SLC35B2 is located in the microsomal membrane and transports PAPS from the cytosol, where it is synthesized, into the Golgi lumen (Kamiyama et al., 2003 [PubMed 12716889]).[supplied by OMIM, Mar 2008],

Additional antibody information

Specificity S35B2 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: S35B2
Collaborator ELK Biotechnology
Partner model ES10769
Target S35B2
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in the placenta, pancreas, mammary gland and skeletal muscle. Weakly or not expressed in colon, heart and prostate.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
S35B2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
47kD
Cellular localization
Golgi apparatus membrane ; Multi-pass membrane protein .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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