His-tag rabbit pAb
His-tag rabbit pAb from ELK Biotechnology Read more ›
Product Description
His-tag rabbit pAb is an ELK Biotechnology antibody for research workflows involving His-tag.
Background
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
Additional antibody information
| Specificity | His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. |
|---|---|
| Validation evidence captions | Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Key Facts
| Target | His-tag |
| Also known as | 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Species independent |
| Observed band | 15kD |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | 6X His synthetic peptide conjugated to KLH. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Species independent |
| Species | Dilution | Notes |
|---|---|---|
| Species independent | Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.