His-tag rabbit pAb from ELK Biotechnology Read more ›
His-tag rabbit pAb is a research-use antibody from ELK Biotechnology for studying His-tag in WB, ELISA workflows with listed reactivity for General. Specifications and supporting documents below should be reviewed when planning the experiment.
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
| Specificity | His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells. |
|---|---|
| Validation evidence captions | Western Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES1076 |
| Target | His-tag |
| Research areas | Antibody Research |
| Reactivity | General |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | 6X His synthetic peptide conjugated to KLH. |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Species independent |
| Species | Dilution | Notes |
|---|---|---|
| Species independent | Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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