Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93011

Cleaved-MPO 89k (A49) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Cleaved-MPO 89k (A49) rabbit pAb from ELK Biotechnology Read more ›

Cleaved-MPO 89k (A49) rabbit pAb
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Cleaved-MPO 89k (A49) rabbit pAb

Product Description

Cleaved-MPO 89k (A49) rabbit pAb is a research-use antibody from ELK Biotechnology for studying MPO in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014],

Additional antibody information

Specificity Cleaved-MPO 89k (A49) Polyclonal Antibody detects endogenous levels of fragment of activated MPO 89k protein resulting from cleavage adjacent to A49.
Validation evidence captions Western Blot analysis of various cells using Cleaved-MPO 89k (A49) Polyclonal Antibody

Key Facts

Product typePrimary Antibody Target: MPO
Collaborator ELK Biotechnology
Partner model ES1070
Target MPO
Research areas>>Drug metabolism - other enzymes, >>Phagosome, >>Neutrophil extracellular trap formation, >>Transcriptional misregulation in cancer, >>Acute myeloid leukemia
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Cleaved-MPO 89k (A49) . at AA range: 40-120
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Leukemia,Leukocyte,Liver,Plasma,Saliva,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
MPO
Also known as
MPO; Myeloperoxidase; MPO
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from Cleaved-MPO 89k (A49) . at AA range: 40-120
Observed band
89kD
Cellular localization
Lysosome.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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