Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92997

MMP-1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MMP-1 rabbit pAb from ELK Biotechnology Read more ›

MMP-1 rabbit pAb
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MMP-1 rabbit pAb

Product Description

MMP-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MMP-1 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

matrix metallopeptidase 1(MMP1) Homo sapiens This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016],

Additional antibody information

Specificity MMP-1 Polyclonal Antibody detects endogenous levels of MMP-1 protein.
Validation evidence captions Western Blot analysis of various cells using MMP-1 Polyclonal Antibody

Key Facts

Product typePrimary Antibody Target: MMP-1
Collaborator ELK Biotechnology
Partner model ES1068
Target MMP-1
Research areas>>PPAR signaling pathway, >>IL-17 signaling pathway, >>Relaxin signaling pathway, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Bladder cancer, >>Rheumatoid arthritis, >>Lipid and atherosclerosis
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from MMP-1 . at AA range: 190-270
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Fibroblast,Ovary,Synovial cell,Synovial membrane,Thyroid,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
MMP-1
Also known as
MMP1; CLG; Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from MMP-1 . at AA range: 190-270
Observed band
55kD
Cellular localization
Secreted, extracellular space, extracellular matrix .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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