Cleaved-Integrin α7 LC (E959) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Integrin α7 LC (E959) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Integrin α7 LC in WB, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
integrin subunit alpha 7(ITGA7) Homo sapiens The protein encoded by this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. They mediate a wide spectrum of cell-cell and cell-matrix interactions, and thus play a role in cell migration, morphologic development, differentiation, and metastasis. This protein functions as a receptor for the basement membrane protein laminin-1. It is mainly expressed in skeletal and cardiac muscles and may be involved in differentiation and migration processes during myogenesis. Defects in this gene are associated with congenital myopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2009],
| Specificity | Cleaved-Integrin α7 LC (E959) Polyclonal Antibody detects endogenous levels of fragment of activated Integrin α7 LC protein resulting from cleavage adjacent to E959. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Integrin α7 LC (E959) Polyclonal Antibody | Western blot analysis of lysates from COS7 cells, treated with etoposide 25uM 1h, using ITGA7 (light chain,Cleaved-Glu959) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1064 |
| Target | Integrin α7 LC |
| Research areas | >>PI3K-Akt signaling pathway, >>Focal adhesion, >>ECM-receptor interaction, >>Regulation of actin cytoskeleton, >>Human papillomavirus infection, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy |
| Reactivity | Human,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ITGA7. AA range:940-989 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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