ABM92947
ASPM rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ASPM rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ASPM rabbit pAb is an ELK Biotechnology antibody for research workflows involving ASPM.
Background
This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011],
Additional antibody information
| Specificity | ASPM Polyclonal Antibody detects endogenous levels of protein. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200 | Western Blot analysis of K-562 cell ,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
Product type
Primary Antibody›
Target: ASPM
| Target | ASPM |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p 1:50-300 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 382kD |
| Cellular localization | Cytoplasm . Cytoplasm, cytoskeleton, spindle . Nucleus . The nuclear-cytoplasmic distribution could be regulated by the availability of calmodulin (By similarity). Localizes to spindle poles during mitosis (PubMed:19690332). Associates with microtubule minus ends (By similarity). . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIF |
| Dilution range | IHC-p 1:50-300 |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 1230-1310 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
Specifications
Target
ASPM
Concentration
1 mg/ml
Working concentration
IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
IHCIF
Immunogen
Synthesized peptide derived from human protein . at AA range: 1230-1310
Observed band
382kD
Cellular localization
Cytoplasm . Cytoplasm, cytoskeleton, spindle . Nucleus . The nuclear-cytoplasmic distribution could be regulated by the availability of calmodulin (By similarity). Localizes to spindle poles during mitosis (PubMed:19690332). Associates with microtubule minus ends (By similarity). .
Dilution range
IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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