Cleaved-Thrombin APII (R327) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Thrombin APII (R327) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Thrombin APII in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],
| Specificity | Cleaved-Thrombin APII (R327) Polyclonal Antibody detects endogenous levels of fragment of activated Thrombin APII protein resulting from cleavage adjacent to R327. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Thrombin APII (R327) Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24H, using THRB (AP2,Cleaved-Arg327) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1055 |
| Target | Thrombin APII |
| Research areas | >>Phospholipase D signaling pathway, >>Neuroactive ligand-receptor interaction, >>Complement and coagulation cascades, >>Platelet activation, >>Regulation of actin cytoskeleton, >>Pathogenic Escherichia coli infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human THRB. AA range:278-327 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed by the liver and secreted in plasma. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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