Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92879

ZFHX4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ZFHX4 rabbit pAb from ELK Biotechnology Read more ›

ZFHX4 rabbit pAb 50µL/100µL
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ZFHX4 rabbit pAb 50µL/100µL

Product Description

ZFHX4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ZFHX4 in WB, IHC, IF workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

disease:A chromosomal aberration involving [ZFHX4] is found in one patient with ptosis. Translocation t(1;8)(p34.3;q21.12).,function:May play a role in neural and muscle differentiation (By similarity). May be involved in transcriptional regulation.,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family.,similarity:Contains 20 C2H2-type zinc fingers.,similarity:Contains 4 homeobox DNA-binding domains.,tissue specificity:Expressed in brain, skeletal muscle and liver. Very low expression in stomach.,

Additional antibody information

Specificity ZFHX4 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product typePrimary Antibody Target: ZFHX4
Collaborator ELK Biotechnology
Partner model ES10485
Target ZFHX4
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,IHC,IF
ConjugationUnconjugated
Dilution rangeWB: 1:500-1000 IHC: 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in brain, skeletal muscle and liver. Very low expression in stomach.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB: 1:500-1000 IHC: 1:200-500
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes

Specifications

Target
ZFHX4
Concentration
1 mg/ml
Working concentration
WB: 1:500-1000 IHC: 1:200-500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIHCIF
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
392kD
Cellular localization
Nucleus .
Dilution range
WB: 1:500-1000 IHC: 1:200-500
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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