ABM92874
Cleaved-MMP-23 (Y79) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Cleaved-MMP-23 (Y79) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Cleaved-MMP-23 (Y79) rabbit pAb is an ELK Biotechnology antibody for research workflows involving MMP-23.
Background
This gene (MMP23B) encodes a member of the matrix metalloproteinase (MMP) family, and it is part of a duplicated region of chromosome 1p36.3. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene belongs to the more telomeric copy of the duplicated region. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Cleaved-MMP-23 (Y79) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-23 protein resulting from cleavage adjacent to Y79. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-MMP-23 (Y79) Polyclonal Antibody | Western blot analysis of lysates from 293 cells, treated with etoposide 25uM 1h, using MMP23 (Cleaved-Tyr79) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: MMP-23
| Target | MMP-23 |
| Also known as | MMP23A; MMP21; MMP23B; MMP21; MMP22; Matrix metalloproteinase-23; MMP-23; Femalysin; MIFR-1; Matrix metalloproteinase-21; MMP-21; Matrix metalloproteinase-22; MMP-22 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 36kD |
| Cellular localization | Endoplasmic reticulum membrane ; Single-pass type II membrane protein . Membrane ; Single-pass type II membrane protein . A secreted form produced by proteolytic cleavage may also exist. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MMP23. AA range:60-109 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
MMP-23
Also known as
MMP23A; MMP21; MMP23B; MMP21; MMP22; Matrix metalloproteinase-23; MMP-23; Femalysin; MIFR-1; Matrix metalloproteinase-21; MMP-21; Matrix metalloproteinase-22; MMP-22
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP23. AA range:60-109
Observed band
36kD
Cellular localization
Endoplasmic reticulum membrane ; Single-pass type II membrane protein . Membrane ; Single-pass type II membrane protein . A secreted form produced by proteolytic cleavage may also exist. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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