Cleaved-MMP-12 (G106) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-MMP-12 (G106) rabbit pAb is a research-use antibody from ELK Biotechnology for studying MMP-12 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016],
| Specificity | Cleaved-MMP-12 (G106) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-12 protein resulting from cleavage adjacent to G106. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-MMP-12 (G106) Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of VEC cells using Cleaved-MMP-12 (G106) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from NIH/3T3 cells, treated with etoposide 25uM 1h, using MMP12 (Cleaved-Glu106) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1043 |
| Target | MMP-12 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MMP12. AA range:87-136 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Found in alveolar macrophages but not in peripheral blood monocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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