Cleaved-MMP-12 (G106) rabbit pAb
Cleaved-MMP-12 (G106) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cleaved-MMP-12 (G106) rabbit pAb is an ELK Biotechnology antibody for research workflows involving MMP-12.
Background
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016],
Additional antibody information
| Specificity | Cleaved-MMP-12 (G106) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-12 protein resulting from cleavage adjacent to G106. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-MMP-12 (G106) Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of VEC cells using Cleaved-MMP-12 (G106) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from NIH/3T3 cells, treated with etoposide 25uM 1h, using MMP12 (Cleaved-Glu106) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | MMP-12 |
| Also known as | MMP12; HME; Macrophage metalloelastase; MME; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 42kD |
| Cellular localization | Secreted, extracellular space, extracellular matrix . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MMP12. AA range:87-136 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.