Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92816

BTF3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

BTF3 rabbit pAb from ELK Biotechnology Read more ›

BTF3 rabbit pAb
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BTF3 rabbit pAb

Product Description

BTF3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying BTF3 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity BTF3 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: BTF3
Collaborator ELK Biotechnology
Partner model ES10374
Target BTF3
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human protein . at AA range: 120-200
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Colon adenocarcinoma,Leukocyte,Skin,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
BTF3
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from human protein . at AA range: 120-200
Observed band
22kD
Cellular localization
Cytoplasm . Nucleus . The heterodimer with NACA is cytoplasmic.
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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