Cleaved-Ephrin-A2 (N188) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Ephrin-A2 (N188) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Ephrin-A2 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq, Jul 2008],
| Specificity | Cleaved-Ephrin-A2 (N188) Polyclonal Antibody detects endogenous levels of fragment of activated Ephrin-A2 protein resulting from cleavage adjacent to N188. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Ephrin-A2 (N188) Polyclonal Antibody | Western blot analysis of lysates from 293 cells, treated with etoposide 25uM 1h, using EFNA2 (Cleaved-Asn188) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1037 |
| Target | Ephrin-A2 |
| Research areas | >>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>PI3K-Akt signaling pathway, >>Axon guidance, >>MicroRNAs in cancer |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EFNA2. AA range:139-188 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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