Cleaved-COL3A1 (G1221) rabbit pAb
Cleaved-COL3A1 (G1221) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cleaved-COL3A1 (G1221) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Collagen III.
Background
collagen type III alpha 1 chain(COL3A1) Homo sapiens This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008],
Additional antibody information
| Specificity | Cleaved-COL3A1 (G1221) Polyclonal Antibody detects endogenous levels of fragment of activated COL3A1 protein resulting from cleavage adjacent to G1221. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-COL3A1 (G1221) Polyclonal Antibody | Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h, using Collagen III alpha1 (Cleaved-Gly1221) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Collagen III |
| Also known as | COL3A1; Collagen alpha-1(III) chain |
| Concentration | 1 mg/ml |
| Working concentration | ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 95kD |
| Cellular localization | Secreted, extracellular space, extracellular matrix . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Platelet activation;>>Relaxin signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Protein digestion and absorption;>>Amoebiasis;>>Diabetic cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
ELISA |
| Dilution range | ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen III alpha1. AA range:1172-1221 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.