Cleaved-COL3A1 (G1221) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-COL3A1 (G1221) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Collagen III in ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
collagen type III alpha 1 chain(COL3A1) Homo sapiens This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008],
| Specificity | Cleaved-COL3A1 (G1221) Polyclonal Antibody detects endogenous levels of fragment of activated COL3A1 protein resulting from cleavage adjacent to G1221. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-COL3A1 (G1221) Polyclonal Antibody | Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h, using Collagen III alpha1 (Cleaved-Gly1221) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1035 |
| Target | Collagen III |
| Research areas | >>Platelet activation, >>Relaxin signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Protein digestion and absorption, >>Amoebiasis, >>Diabetic cardiomyopathy |
| Reactivity | Human,Rat,Mouse |
| Applications | ELISA |
| Conjugation | Unconjugated |
| Dilution range | ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen III alpha1. AA range:1172-1221 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Colon carcinoma,Liver,Placenta,Skin fibroblast, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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