Cleaved-Cathepsin D LC (G65) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Cathepsin D LC (G65) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cathepsin D in WB, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. [provided by RefSeq, Nov 2015],
| Specificity | Cleaved-Cathepsin D LC (G65) Polyclonal Antibody detects endogenous levels of fragment of activated Cathepsin D LC protein resulting from cleavage adjacent to G65. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Cathepsin D LC (G65) Polyclonal Antibody | Western blot analysis of lysates from COS7 cells, treated with etoposide 25uM 1h, using CATD (light chain,Cleaved-Gly65) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1027 |
| Target | Cathepsin D |
| Research areas | >>Sphingolipid signaling pathway, >>Autophagy - animal, >>Lysosome, >>Apoptosis, >>Estrogen signaling pathway, >>Tuberculosis, >>Diabetic cardiomyopathy |
| Reactivity | Human,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CATD. AA range:46-95 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in the aorta extracellular space (at protein level) (PubMed:20551380). Expressed in liver (at protein level) (PubMed:1426530). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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