Cleaved-Caspase-7 p20 (D198) rabbit pAb
Cleaved-Caspase-7 p20 (D198) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cleaved-Caspase-7 p20 (D198) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Caspase-7.
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
Additional antibody information
| Specificity | Cleaved-Caspase-7 p20 (D198) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-7 p20 protein resulting from cleavage adjacent to D198. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Caspase-7 p20 (D198) Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using Caspase 7 (p20,Cleaved-Asp198) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Caspase-7 |
| Also known as | CASP7; MCH3; Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 20kD |
| Cellular localization | Cytoplasm. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Apoptosis;>>Apoptosis - multiple species;>>TNF signaling pathway;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Pathogenic Escherichia coli infection;>>Salmonella infection;>>Pertussis;>>Legionellosis;>>Pathways in cancer;>>Lipid and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 7. AA range:149-198 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.