Cleaved-Caspase-7 p20 (D198) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Caspase-7 p20 (D198) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Caspase-7 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
| Specificity | Cleaved-Caspase-7 p20 (D198) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-7 p20 protein resulting from cleavage adjacent to D198. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Caspase-7 p20 (D198) Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using Caspase 7 (p20,Cleaved-Asp198) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1023 |
| Target | Caspase-7 |
| Research areas | >>Apoptosis, >>Apoptosis - multiple species, >>TNF signaling pathway, >>Non-alcoholic fatty liver disease, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Pathogenic Escherichia coli infection, >>Salmonella infection, >>Pertussis, >>Legionellosis, >>Pathways in cancer, >>Lipid and atherosclerosis |
| Reactivity | Human,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 7. AA range:149-198 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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