Cleaved-Caspase-5 p20 (D121) rabbit pAb
Cleaved-Caspase-5 p20 (D121) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cleaved-Caspase-5 p20 (D121) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Caspase-5.
Background
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010],
Additional antibody information
| Specificity | Cleaved-Caspase-5 p20 (D121) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-5 p20 protein resulting from cleavage adjacent to D121. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Caspase-5 p20 (D121) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from HeLa and A549 cells, treated with etoposide 25uM 24h, using Caspase 5 (p20,Cleaved-Asp121) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Caspase-5 |
| Also known as | CASP5; ICH3; Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 22kD |
| Cellular localization | neuron projection,neuronal cell body,IPAF inflammasome complex,NLRP1 inflammasome complex,NLRP3 inflammasome complex,AIM2 inflammasome complex, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>NOD-like receptor signaling pathway;>>Salmonella infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 5. AA range:102-151 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.