Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92694

Cleaved-C1s HC (R437) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Cleaved-C1s HC (R437) rabbit pAb from ELK Biotechnology Read more ›

Cleaved-C1s HC (R437) rabbit pAb 50µL/100µL
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Cleaved-C1s HC (R437) rabbit pAb 50µL/100µL

Product Description

Cleaved-C1s HC (R437) rabbit pAb is a research-use antibody from ELK Biotechnology for studying C1S in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq, Mar 2009],

Additional antibody information

Specificity Cleaved-C1s HC (R437) Polyclonal Antibody detects endogenous levels of fragment of activated C1s HC protein resulting from cleavage adjacent to R437.
Validation evidence captions Western Blot analysis of various cells using Cleaved-C1s HC (R437) Polyclonal Antibody | Western blot analysis of lysates from 293 and HeLa cells, treated with etoposide 25uM 1h, using C1S (heavy chain,Cleaved-Arg437) Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: C1S
Collaborator ELK Biotechnology
Partner model ES1014
Target C1S
Research areas>>Complement and coagulation cascades, >>Pertussis, >>Staphylococcus aureus infection, >>Coronavirus disease - COVID-19, >>Systemic lupus erythematosus
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human C1S. AA range:388-437
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Liver,Peripheral blood leukocyte,Plasma,PNS,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
C1S
Also known as
C1S; Complement C1s subcomponent; C1 esterase; Complement component 1 subcomponent s
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human C1S. AA range:388-437
Observed band
47+76kD
Cellular localization
extracellular region,extracellular exosome,blood microparticle,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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