Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92663

Adiponectin rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Adiponectin rabbit pAb from ELK Biotechnology Read more ›

Adiponectin rabbit pAb 50µL/100µL
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Adiponectin rabbit pAb 50µL/100µL

Product Description

Adiponectin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Adiponectin in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

adiponectin, C1Q and collagen domain containing(ADIPOQ) Homo sapiens This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010],

Additional antibody information

Specificity Adiponectin Polyclonal Antibody detects endogenous levels of Adiponectin protein.
Validation evidence captions Western Blot analysis of various cells using Adiponectin Polyclonal Antibody diluted at 1:2000

Key Facts

Product typePrimary Antibody Target: Adiponectin
Collaborator ELK Biotechnology
Partner model ES1011
Target Adiponectin
Research areas>>PPAR signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Adipocytokine signaling pathway, >>Type II diabetes mellitus, >>Non-alcoholic fatty liver disease, >>Alcoholic liver disease
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from Adiponectin . at AA range: 10-90
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Synthesized exclusively by adipocytes and secreted into plasma.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Adiponectin
Also known as
ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte; C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from Adiponectin . at AA range: 10-90
Observed band
32kD
Cellular localization
Secreted .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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