RAIN rabbit pAb
RAIN rabbit pAb from ELK Biotechnology Read more ›
Product Description
RAIN rabbit pAb is an ELK Biotechnology antibody for research workflows involving RAIN.
Background
function:May act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation.,similarity:Contains 1 dilute domain.,similarity:Contains 1 Ras-associating domain.,subcellular location:Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS.,subunit:Interacts with Ras family members that have been activated by GTP binding. Interacts with HRAS, RAP1A, RAP2, RRAS, RAF1 and RRAS2.,tissue specificity:Highly expressed in heart. Detected at lower levels in placenta and pancreas.,
Additional antibody information
| Specificity | RAIN Polyclonal Antibody detects endogenous levels of protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of Hela lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
| Target | RAIN |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 ELISA 1:5000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 105kD |
| Cellular localization | Cytoplasm, perinuclear region . Golgi apparatus, Golgi stack . Associated with perinuclear vesicles. Is recruited to Golgi stacks by activated HRAS. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000 ELISA 1:5000-20000 |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 620-700 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 ELISA 1:5000-20000 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.