Collaborator ELK Biotechnology
Recombinant ProteinsISO 9001:2015
ABM100857

Recombinant Vibriocholerae Toxin B

ISO 9001|ABMIUM Verified™|Protein

Recombinant Vibriocholerae Toxin B from ELK Biotechnology Read more ›

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Product Description

Recombinant Vibriocholerae Toxin B is a research-use recombinant protein from ELK Biotechnology preparation representing Recombinant Vibriocholerae Toxin B supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Vibriocholerae Toxin B is produced by our E.coli expression system and the target gene encoding Thr22-Asn124 is expressed.

Background

Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. Cholera enterotoxin subunit B (CTXB) pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Key Facts

Product typeRecombinant Proteins Target: Recombinant Vibriocholerae Toxin B
Collaborator ELK Biotechnology
Partner model EPT133
Target Recombinant Vibriocholerae Toxin B
Research areasMolecular Biology
Reactivity Product QC:C
Applications Protein research
Assay type Recombinant protein
Purity >95% by SDS-PAGE
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryPurityEndotoxinBioactivity
Product QC

Specifications

Target
Recombinant Vibriocholerae Toxin B
Also known as
Cholera enterotoxin subunit B;Cholera enterotoxin B chain;Cholera enterotoxin gamma chain;Choleragenoid;ctxB;toxB
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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