Recombinant Mouse TfR (N-8His) from ELK Biotechnology Read more ›
Recombinant Mouse TfR (N-8His) is a research-use recombinant protein from ELK Biotechnology preparation representing TfR supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.
Recombinant Mouse Transferrin Receptor Protein 1 is produced by our Mammalian expression system and the target gene encoding Cys89-Phe763 is expressed with a 8His tag at the N-terminus.
Transferrin receptor protein 1 (TFRC) belongs to the peptidase M28 family that is synthesized as a 172 amino acid (aa). TFRC regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. It binds one transferrin or HFE molecule per subunit and binds the HLA class II histocompatibility antigen, DR1. It Interacts with SH3BP3 and STEAP3, facilitates TFRC endocytosis in erythroid precursor cells. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. It positively regulates T and B cell proliferation through iron uptake.
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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| Delivery condition | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| Collaborator | ELK Biotechnology |
| Partner model | EPT081 |
| Target | TfR |
| Research areas | Molecular Biology |
| Reactivity | Product QC:C |
| Applications | Protein research |
| Assay type | Recombinant protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Category | Purity | Endotoxin | Bioactivity |
|---|---|---|---|
| Product QC |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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