Collaborator ELK Biotechnology
Recombinant ProteinsISO 9001:2015
ABM100795

Recombinant S. hygroscopicus BAR

ISO 9001|ABMIUM Verified™|Protein

Recombinant S. hygroscopicus BAR from ELK Biotechnology Read more ›

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Product Description

Recombinant S. hygroscopicus BAR is a research-use recombinant protein from ELK Biotechnology preparation representing Recombinant S. hygroscopicus BAR supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Streptomyces Hygroscopicus Phosphinothricin N-acetyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.

Background

Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Key Facts

Product typeRecombinant Proteins Target: Recombinant S. hygroscopicus BAR
Collaborator ELK Biotechnology
Partner model EPT071
Target Recombinant S. hygroscopicus BAR
Research areasMolecular Biology
Reactivity Product QC:C
Applications Protein research
Assay type Recombinant protein
Purity >95% by SDS-PAGE
Endotoxin Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
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Validation Matrix

1 category
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
CategoryPurityEndotoxinBioactivity
Product QC

Specifications

Target
Recombinant S. hygroscopicus BAR
Also known as
Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar
Purity
>95% by SDS-PAGE
Form / buffer
Lyophilized

Storage & Stability

Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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